Identification, Screening and Antibacterial Mechanism Analysis of Novel Antimicrobial Peptides from Sturgeon (Acipenser ruthenus) Spermary

Fish is an important source of antimicrobial peptides. This study aimed to identify and screen antibacterial peptides with excellent activity derived from sturgeon spermary (SSPs) analyze their mechanism. Liquid chromatography-mass spectrometry/mass spectrometry methods were used peptide sequences, computational prediction tool molecular docking for virtual screening peptides, finally, candidate synthesized by solid-phase synthesis method. The results demonstrate that SSPs have inhibitory against Escherichia coli rate 76.46%. Most parts the (Acipenser ruthenus) histones, coverage histone H2B was highest (45%). Two novel (NDEELNKLM RSSKRRQ) obtained in silico tools docking, which may interact DNA gyrase dihydrofolate reductase E. forming salt bridges hydrogen bonds. Compared individual effect significantly improved mixing two equal proportions. change permeability cell membranes exert inhibiting metabolic process nucleic acids.